B22 overexpression of MyoD Differentiated 24h H3.3
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
sample_name
DRS000208
sample comment
source: B22 cells were generated previously described in de la Serna et al. (2000) Mol. Cell. Biol. 20, 2839-2851
Sequenced DNA Library
library_name
B22_overexpression of MyoD_Differentiated_24h_H3.3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP analyses were performed as described in Pasini et al. (2008). Genes Dev 22, 1345-1355. DNA from ChIP experiments was pooled and prepared for sequencing with the Illumina ChIPSeq Sample Prep kit. DNA libraries were quantified using a bioanalyzer (Agilent) and diluted to 5-10 pM. Diluted libraries were used directly for cluster generation and sequencing analysis using the Genome Analyzer IIx (Illumina) following the protocol of the manufacturer. Basecalling and mapping of the 36 bp sequences were done using illumina RTA SCS2.6 allowing up to two mismatches in the first 35 bases of a read against mouse genomes July 2007 release (mm9).