Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Stat92E

Cell type

Cell type Class
Adult
Cell type
Intestines
NA
NA

Attributes by original data submitter

Sample

source_name
adult intestines
strain background
W1118
fly genotypes
esgts>Upd and STAT92E
developmental stage
adult
cultural condition
29? for 10 days
tissue
intestines
chip antibody
mouse-raised STAT92E antibody (self-made); 100-150aa polypeptides of STAT92E protein chosen as the antigen

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
About 400 Drosophila adult midguts(with the hindgut and Malpighian excluded) were dissected in ice-cold PBS, and then cross-linked with 1% formaldehyde for 15 minutes. After washing process to remove the formaldehyde, intestinal tissue was lysed with RIPA buffer which contains 1% SDS on ice for 30 minutes. The sonication of chromatin was performed using the Covaris (AFA) system with 3% power output for 5 minutes each on 100μl lysates.STAT92E-bound chromatin fragments were enriched by immunoprecipitation with mouse raised STAT92E antibody. Most chromatin fragments resulting from sonication occurred between 200 and 400 bp.The process of dilution, antibody incubation, protein G pull down, beads washing, DNA complex elution, de-link, RNAse A / protease K digestion and DNA extraction all with normal ChIP protocols.The high throughput sequencing process was carried out using the Illumina solexa system. ChIP and input libraries were prepared using NEXTflex™ Illumina ChIP-Seq Library Prep Kit (Illumina) according to the manufacturer’s protocol. The indexed libraries were pooled together, and sequenced using the Illumina HiSeq 2000 (Illumina) instrument.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
8156820
Reads aligned (%)
54.5
Duplicates removed (%)
86.2
Number of peaks
622 (qval < 1E-05)

dm6

Number of total reads
8156820
Reads aligned (%)
54.0
Duplicates removed (%)
87.9
Number of peaks
136 (qval < 1E-05)

Base call quality data from DBCLS SRA