Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
mix stage culture
developmental stage
Mix Culture
temperature
20 °C
strain
daf-2 (e1370)
chip antibody
input

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Worms were harvested from plates with 1 X PBS buffer and washed four times. The compact worm slurry (250 µl) was resuspended in 4 ml cross-linking buffer (1% formaldehyde in 1 X PBS) followed by homogenization with 7 ml glass Dounce homogenizer. A total of 5 ng of DNA was used as a starting material for library preparation according to the Illumina, Inc. ( San Diego, CA, USA) protocol, "Preparing Samples for ChIP Sequencing of DNA".

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

ce10

Number of total reads
17254347
Reads aligned (%)
98.6
Duplicates removed (%)
78.3
Number of peaks
1714 (qval < 1E-05)

ce11

Number of total reads
17254347
Reads aligned (%)
98.6
Duplicates removed (%)
78.3
Number of peaks
1719 (qval < 1E-05)

Base call quality data from DBCLS SRA