Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Clamp

Cell type

Cell type Class
Embryo
Cell type
Embryos
NA
NA

Attributes by original data submitter

Sample

source_name
embryo
antibody
CLAMP
nuclear cycle
14
Sex
female
tissue
embryo

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
We performed 0- to 4-h timed lays and collected and fixed embryos according to Blythe and Wieschaus (2015). We then hand-sorted embryos using a Zeiss Discovery.V8 microscope under GFP excitation using an X-CITE 120Q stereo light source. We pooled 200 (NC 11–14) to 400 (NC < 11) embryos and performed ChIP as in Blythe and Wieschaus (2015) using 3 μL of rabbit anti-CLAMP antibody per sample.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
21424868
Reads aligned (%)
43.6
Duplicates removed (%)
38.4
Number of peaks
949 (qval < 1E-05)

dm6

Number of total reads
21424868
Reads aligned (%)
43.4
Duplicates removed (%)
41.4
Number of peaks
2328 (qval < 1E-05)

Base call quality data from DBCLS SRA