Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
Whoel animal extract
strain
HML214
tag
GFP
antibody
n/a

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Staged worms were washed twice with M9 buffer and freezed in liquid nitrogen. Frozan worms were crushed using a pestle and a mortar and resuspended in Chip buffer. Extracts were sonicated and precleared before ChIP experiments. Libraires were made followinf the recommended Illumina protocol for TruSeq Chip sample preparation guide (part #15023092 Rev A, August 2012).

Sequencing Platform

instrument_model
Illumina MiSeq

ce10

Number of total reads
4907316
Reads aligned (%)
82.3
Duplicates removed (%)
67.4
Number of peaks
152 (qval < 1E-05)

ce11

Number of total reads
4907316
Reads aligned (%)
82.3
Duplicates removed (%)
67.4
Number of peaks
152 (qval < 1E-05)

Base call quality data from DBCLS SRA