Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Kasumi-1 Cells
cell line
Kasumi-1
cell type
Acute Myeloid Leukemia
chip antibody
H3K27ac (Millipore, 07-360)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
SimpleChIP Enzymatic Chromatin IP kit (Cell Signaling Technology) was used for chromatin immunoprecipitation per manufacturer's instructions. Libraries were prepared using E7645S NEBNext(r) Ultra(tm) II DNA Library Prep Kit for Illumina® according to manufacturer's instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
32427519
Reads aligned (%)
84.2
Duplicates removed (%)
14.2
Number of peaks
22581 (qval < 1E-05)

hg38

Number of total reads
32427519
Reads aligned (%)
85.6
Duplicates removed (%)
13.3
Number of peaks
22701 (qval < 1E-05)

Base call quality data from DBCLS SRA