Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Clamp

Cell type

Cell type Class
Larvae
Cell type
3rd instar
NA
NA

Attributes by original data submitter

Sample

source_name
Female Melanogaster CLAMP ChIPseq
genotype
wild type
chip antibody
anti-CLAMP (SDix, Cat# 4988.00.02, Lot# G3131-080A02; PMID 22844249)
developmental stage
3rd instar larvae

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard ChIP seq protocol - larvae were homogenized in liquid nitrogen followed by lysis and sonication, chromatin was immunoprecipitated with CLAMP antibody. ChIP-seq libraries were prepared with the illumina TruSeq DNA Prep kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
5639720
Reads aligned (%)
79.2
Duplicates removed (%)
77.6
Number of peaks
523 (qval < 1E-05)

dm6

Number of total reads
5639720
Reads aligned (%)
79.0
Duplicates removed (%)
78.9
Number of peaks
679 (qval < 1E-05)

Base call quality data from DBCLS SRA