Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
mix stage culture
developmental stage
Mix Culture
temperature
20 °C
genotype
Wild-type (N2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Worms were harvested from plates with 1 X PBS buffer and washed four times. The compact worm slurry (250 µl) was resuspended in 4 ml cross-linking buffer (1% formaldehyde in 1 X PBS) followed by homogenization with 7 ml glass Dounce homogenizer. A total of 5 ng of DNA was used as a starting material for library preparation according to the Illumina, Inc. ( San Diego, CA, USA) protocol, "Preparing Samples for ChIP Sequencing of DNA".

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

ce11

Number of total reads
37754539
Reads aligned (%)
53.0
Duplicates removed (%)
62.8
Number of peaks
8524 (qval < 1E-05)

ce10

Number of total reads
37754539
Reads aligned (%)
53.0
Duplicates removed (%)
62.8
Number of peaks
8288 (qval < 1E-05)

Base call quality data from DBCLS SRA