Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Spermatocytes

MeSH Description

Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.

Sample

source_name

spermatocytes

antibody

none

cell type

spermatocytes

age

12 days post-partum

strain

C57BL/6J

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were isolated from cells using hypotonic lysis. Chromatin was fragmented either by MNase digestion. After high speed spin, soluble chromatin was removed and used for ChIP. Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

31942100

Reads aligned (%)

95.8

Duplicates removed (%)

6.0

Number of peaks

215 (qval < 1E-05)

mm9

Number of total reads

31942100

Reads aligned (%)

95.7

Duplicates removed (%)

6.1

Number of peaks

160 (qval < 1E-05)