Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
prostate cancer cells
cell line
LNCaP-abl
cell type
androgen-independent prostate cancer cells
passages
61-64
chip antibodies
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with specific antibodies. Libraries were prepared using ThruPLEX-FD Prep Kit (Rubicon Genomics). The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina NextSeq 500 system following the manufacturer's protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
48344017
Reads aligned (%)
81.1
Duplicates removed (%)
74.2
Number of peaks
4138 (qval < 1E-05)

hg19

Number of total reads
48344017
Reads aligned (%)
79.2
Duplicates removed (%)
77.7
Number of peaks
4368 (qval < 1E-05)

Base call quality data from DBCLS SRA