GSM1656410: E2F1 ChIPSeq abl; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
E2F1
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
prostate cancer cells
cell line
LNCaP-abl
cell type
androgen-independent prostate cancer cells
passages
61-64
chip antibodies
Santa Cruz Biotechnology (sc-193) and BD Pharmingen (554213)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with specific antibodies. Libraries were prepared using ThruPLEX-FD Prep Kit (Rubicon Genomics). The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina NextSeq 500 system following the manufacturer's protocols.