Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

HEK293

cell description

embryonic kidney

prdm9 allele

empty vector

ChIP

H3K4me3

antibody manufacturer

Millipore/EMD

antibody catalog number

07-473

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were isolated from cells using hypotonic lysis. Chromatin was fragmented by Mnase digestion. After high speed spin soluble chromatin was removed and used for ChIP Kapa Hyper Prep Kits (Kapa Biosystems)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

50003235

Reads aligned (%)

89.5

Duplicates removed (%)

11.5

Number of peaks

41680 (qval < 1E-05)

hg19

Number of total reads

50003235

Reads aligned (%)

88.9

Duplicates removed (%)

11.3

Number of peaks

42778 (qval < 1E-05)