GSM1652707: Input N2 YA Rep1; Caenorhabditis elegans; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Adult
Cell type
Young adult
NA
NA
Attributes by original data submitter
Sample
source_name
whole worms
strain
N2
developmental stage
YA
antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Frozen, ground, and crosslinked larva samples were resuspended in FA buffer+sarkosyl+protease inhibitors (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1 % sodium deoxycholate; 150 mM NaCl, 0.1% sarkosyl), and sonicated to obtain chromatin fragments of majority between 200-800 bp. 1-2 mg of extract and 3-5 ug of antibody was used per ChIP. Half of the ChIP DNA and 10-20 ng of input DNA were ligated to Illumina multiplexed adapters and amplified by PCR. Library DNA between 250-500 bp in size was gel purified.