Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H4K20me1

Cell type

Cell type Class
Larvae
Cell type
L3
NA
NA

Attributes by original data submitter

Sample

source_name
whole worms
strain
MT14911
developmental stage
L3
matching input raw data file
Input_SET4_L3_Rep1_1.fastq.gz,Input_SET4_L3_Rep1_2.fastq.gz
antibody
H4K20me1 Abcam (ab9051)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Frozen, ground, and crosslinked larva samples were resuspended in FA buffer+sarkosyl+protease inhibitors (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1 % sodium deoxycholate; 150 mM NaCl, 0.1% sarkosyl), and sonicated to obtain chromatin fragments of majority between 200-800 bp. 1-2 mg of extract and 3-5 ug of antibody was used per ChIP. Half of the ChIP DNA and 10-20 ng of input DNA were ligated to Illumina multiplexed adapters and amplified by PCR. Library DNA between 250-500 bp in size was gel purified.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
10807193
Reads aligned (%)
93.0
Duplicates removed (%)
6.7
Number of peaks
597 (qval < 1E-05)

ce10

Number of total reads
10807193
Reads aligned (%)
93.0
Duplicates removed (%)
6.7
Number of peaks
588 (qval < 1E-05)

Base call quality data from DBCLS SRA