Curated Sample Data


Genome
ce10
Antigen Class
Histone
Antigen
H4K20me1
Cell type Class
Larvae
Cell type
L3

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
whole worms
strain
CB428
developmental stage
L3
matching input raw data file
Input_CB428_L3_Rep2_1.fastq.gz,Input_CB428_L3_Rep2_2.fastq.gz
antibody
H4K20me1 Abcam (ab9051)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Frozen, ground, and crosslinked larva samples were resuspended in FA buffer+sarkosyl+protease inhibitors (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1 % sodium deoxycholate; 150 mM NaCl, 0.1% sarkosyl), and sonicated to obtain chromatin fragments of majority between 200-800 bp. 1-2 mg of extract and 3-5 ug of antibody was used per ChIP. Half of the ChIP DNA and 10-20 ng of input DNA were ligated to Illumina multiplexed adapters and amplified by PCR. Library DNA between 250-500 bp in size was gel purified.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
11533899
Reads aligned (%)
66.7
Duplicates removed (%)
7.2
Number of peaks
597 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA