Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

Crosslinked MNase digested chromatin

cell line

K562 cells

antibody

CTCF (Millipore catalog #07-729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Human K562 cells were crosslinked using formaldehyde for 10 minutes at room temperature. Chromatin was fragmented using MNase and then sonicated to ensure complete extraction of chromatin and associated proteins. Chromatin was immunoprecipitated using an antibody against CTCF. DNA was extracted from the immunoprecipitated chromatin and subjected to Illumina (Solexa) sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

123439465

Reads aligned (%)

96.5

Duplicates removed (%)

31.6

Number of peaks

14563 (qval < 1E-05)

hg19

Number of total reads

123439465

Reads aligned (%)

96.2

Duplicates removed (%)

31.9

Number of peaks

14403 (qval < 1E-05)