GSM1644676: UNC0638 96hrs input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293T cells
cell line
HEK293T
treatment
UNC0638
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed using the ChIP-IT High Sensitivity kit (Active Motif #53040) according to manufacturer’s instructions except that Input samples were purified by phenol:chloroform extraction. The following antibodies were used: anti-H3K9me2 (Abcam #ab1220), anti-G9a (Abcam #ab40542), and Rabbit IgG (Jackson Immunoresearch #011-000-003). ChIP samples were quantitated using the Qubit 2.0 Fluorometer (Invitrogen). A portion of each sample was confirmed by qPCR. The remainder of the sample was submitted for high throughput sequencing. Libraries were prepared for high-throughput using the Illumina TruSeq kit as per manufacturer’s recommendations.