Antigen

Antigen Class

TFs and others

Antigen

EHMT2

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

HEK293T cells

cell line

HEK293T

treatment

siWIZ

chip antibody

G9a

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was performed using the ChIP-IT High Sensitivity kit (Active Motif #53040) according to manufacturer’s instructions except that Input samples were purified by phenol:chloroform extraction. The following antibodies were used: anti-H3K9me2 (Abcam #ab1220), anti-G9a (Abcam #ab40542), and Rabbit IgG (Jackson Immunoresearch #011-000-003). ChIP samples were quantitated using the Qubit 2.0 Fluorometer (Invitrogen). A portion of each sample was confirmed by qPCR. The remainder of the sample was submitted for high throughput sequencing. Libraries were prepared for high-throughput using the Illumina TruSeq kit as per manufacturer’s recommendations.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

52810982

Reads aligned (%)

98.5

Duplicates removed (%)

14.2

Number of peaks

4246 (qval < 1E-05)

hg19

Number of total reads

52810982

Reads aligned (%)

98.1

Duplicates removed (%)

14.8

Number of peaks

4258 (qval < 1E-05)