Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EHMT2

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293T cells
cell line
HEK293T
treatment
DMSO
chip antibody
G9a

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed using the ChIP-IT High Sensitivity kit (Active Motif #53040) according to manufacturer’s instructions except that Input samples were purified by phenol:chloroform extraction. The following antibodies were used: anti-H3K9me2 (Abcam #ab1220), anti-G9a (Abcam #ab40542), and Rabbit IgG (Jackson Immunoresearch #011-000-003). ChIP samples were quantitated using the Qubit 2.0 Fluorometer (Invitrogen). A portion of each sample was confirmed by qPCR. The remainder of the sample was submitted for high throughput sequencing. Libraries were prepared for high-throughput using the Illumina TruSeq kit as per manufacturer’s recommendations.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
83524950
Reads aligned (%)
90.6
Duplicates removed (%)
6.8
Number of peaks
2228 (qval < 1E-05)

hg38

Number of total reads
83524950
Reads aligned (%)
92.3
Duplicates removed (%)
5.6
Number of peaks
2517 (qval < 1E-05)

Base call quality data from DBCLS SRA