GSM1645703: OCI-Ly7 rep1 input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
OCI-LY1
NA
NA
Attributes by original data submitter
Sample
source_name
lymphoma cell line
cell line
OCI-Ly1
cell type
lymphoma B cells
antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sheared to 200-400bp, incubated with H3K4me2 antibody (abcam 32356) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr. Final chormatin was purified using Qiagen PCR purification columns. Illimunia Truseq ChIPseq protocol