Curated Sample Data


Genome
hg19
Antigen Class
Histone
Antigen
H3K4me2
Cell type Class
Blood
Cell type
OCI-LY1

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
lymphoma cell line
cell line
OCI-Ly1
cell type
lymphoma B cells
antibody
H3K4me2 antibody (abcam 32356)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sheared to 200-400bp, incubated with H3K4me2 antibody (abcam 32356) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr. Final chormatin was purified using Qiagen PCR purification columns. Illimunia Truseq ChIPseq protocol

Platform Information


instrument_model
Illumina Genome Analyzer IIx

External Database Query

Logs in read processing pipeline


Number of total reads
39577437
Reads aligned (%)
98.8
Duplicates removed (%)
9.5
Number of peaks
70394 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA