B-lymphoid immortalized with Abelson murine leukemia virus
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) on clonal cell line Abl.1 was performed as previously described (Nag et al. Elife. 2013 Dec 31;2:e01256). Briefly, cells were fixed with 1% formaldehyde for 5 minutes at 37°C. Fragmentation was performed using Covaris sonicator. An aliquot of sheared chromatin was saved as input control. Immunoprecipitation was performed with Anti-H3K27me3 antibody (ABE44 Millipore, Billerica, MA) and anti-H3K36me3 antibody (AB9050, Abcam, Cambridge, MA and UK), using Protein-A Sepharose beads. ChIP-Seq libraries were prepared using NEBNext ChIP-Seq library prep reagent set for Illumina (NEB E6200S) following manufacturer’s instructions. Barcoded libraries were sequenced using Illumina HiSeq platform (SE50).