Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Srf

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
bone marrow-derived macrophages
strain
C57BL/6
genotype
wildtype
antibody
SRF
stimulus
lipid A
time
60

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Purified nuclei from BMDMs were lysed and chromatin was sheared using a Bioruptor prior to immunoprecipitation with anti-RelA or anti-SRF antibodies RelA libraries were constructed using Illumina's TruSeq ChIP Sample Prep Kit and SRF libraries were constructed using Kapa's LTP Library Preparation kit, both according to manufactuer's instruction.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
31025578
Reads aligned (%)
88.6
Duplicates removed (%)
17.6
Number of peaks
851 (qval < 1E-05)

mm9

Number of total reads
31025578
Reads aligned (%)
88.3
Duplicates removed (%)
17.6
Number of peaks
920 (qval < 1E-05)

Base call quality data from DBCLS SRA