Antigen

Antigen Class

Histone

Antigen

H3S10ph

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

cell type

Embryonic stem cells

strain

129P2/Ola

antibody

ab14955, abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For each sample, 1 x 10^6 cells were cross-linked with 1% PFA and cell nuclei were prepared using a swelling buffer (25 mM Hepes, pH 7.8, 1 mM MgCl2, 10 mM KCl, 0.1% NP-40, 1 mM DTT). For histone modifiacations, chromatin was sheared to mononucleosomal fragments. For RNA-seq RNA was extracted and treated with RiboZero followed by DNaseI digestion. Libraries were prepared according to Illumina standard protocols with internal barcodes.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

24238695

Reads aligned (%)

90.7

Duplicates removed (%)

84.4

Number of peaks

5061 (qval < 1E-05)

mm9

Number of total reads

24238695

Reads aligned (%)

89.4

Duplicates removed (%)

84.5

Number of peaks

5038 (qval < 1E-05)