GSM1639861: Syn ESC H3S10p ChIPseq 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3S10ph
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Embryonic stem cells
cell type
Embryonic stem cells
strain
129P2/Ola
antibody
ab14955, abcam
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For each sample, 1 x 10^6 cells were cross-linked with 1% PFA and cell nuclei were prepared using a swelling buffer (25 mM Hepes, pH 7.8, 1 mM MgCl2, 10 mM KCl, 0.1% NP-40, 1 mM DTT). For histone modifiacations, chromatin was sheared to mononucleosomal fragments. For RNA-seq RNA was extracted and treated with RiboZero followed by DNaseI digestion. Libraries were prepared according to Illumina standard protocols with internal barcodes.