Chromatin Immunoprecipitation was performed using Cell Signaling Technology SimpleChIP Plus Enzymatic Chromatin IP Kit (Magnentic Beads) #9005. For crosslinking, 260 ul formaldehyde 37% solution was added to 10 ml medium of each 10 cm dish, then samples were prepared according to the manufacturer’s instructions. H3K27Me3 antibody (Cell signaling technologies # 9733) was used for the immunoprecipitation. After the procedure chiped DNA was retrieved at a concentration of 7.28ng/ul. As control, the 2% input sample was used. A volume of 10 microlitters of each DNA solution was submitted for chip sequencing to Otogenetics. library construction was performed by otogenetics