Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adult
Cell type
Adult
NA
NA

Attributes by original data submitter

Sample

source_name
whole animal
genotype
fer-1;him-8
antibody
none
tissue
whole animal
developmental stage
adult

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was disrupted and chromatin was sheared with a probe sonicator, and histone-DNA complexes were isolated with antibody. DNA libraries were generated using the NEBNext® ChIP-seq Library Prep Master Mix Set for Illumina® (NEB #E6240) following the manufacturer’s protocol and using Qiagen column purification.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
64271618
Reads aligned (%)
67.7
Duplicates removed (%)
25.5
Number of peaks
0 (qval < 1E-05)

ce10

Number of total reads
64271618
Reads aligned (%)
67.7
Duplicates removed (%)
25.5
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA