Curated Sample Data


Genome
ce10
Antigen Class
Histone
Antigen
H3K9me2
Cell type Class
Adult
Cell type
Adult

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
whole animal
genotype
fer-1
antibody
H3K9me2 (Abcam ab1220, lot#GR98362-1, 0.8mg/ml)
tissue
whole animal
developmental stage
adult

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was disrupted and chromatin was sheared with a probe sonicator, and histone-DNA complexes were isolated with antibody. DNA libraries were generated using the NEBNext® ChIP-seq Library Prep Master Mix Set for Illumina® (NEB #E6240) following the manufacturer’s protocol and using Qiagen column purification.

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
19526266
Reads aligned (%)
82.7
Duplicates removed (%)
71.3
Number of peaks
5312 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA