Sample information curated by ChIP-Atlas

Antigen

Antigen Class
DNase-seq
Antigen
DNase-Seq

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293T_HBG112_cas9 cell line
transfection
dcas9 targetting HBG1/2

Sequenced DNA Library

library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
Cells were Lysed and nuclei were digested according to the publication PMID: 20150147. Libraries were prepared according to the publication PMID: 20150147, except that 5' of Oligo1b was phosphated to enhance ligation.libraries were sequenced on a Illumina HiSeq 2000, with 4 barcodes per lane. Reads were aligned tohuman RefSeq using BWA .

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
30000886
Reads aligned (%)
0.0
Duplicates removed (%)
28.1
Number of peaks
0 (qval < 1E-05)

hg19

Number of total reads
30000886
Reads aligned (%)
0.0
Duplicates removed (%)
30.9
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA