GSM1635911: HEK293T HBG112 cas9 replicate 5; Homo sapiens; DNase-Hypersensitivity
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
DNase-seq
Antigen
DNase-Seq
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293T_HBG112_cas9 cell line
transfection
dcas9 targetting HBG1/2
Sequenced DNA Library
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
Cells were Lysed and nuclei were digested according to the publication PMID: 20150147. Libraries were prepared according to the publication PMID: 20150147, except that 5' of Oligo1b was phosphated to enhance ligation.libraries were sequenced on a Illumina HiSeq 2000, with 4 barcodes per lane. Reads were aligned tohuman RefSeq using BWA .