Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryo

Cell type

Embryonic brains

NA

NA

Sample

source_name

e12.5 embryonic forebrain, input

strain/background

CD-1

developmental stage

embryo

age

e12.5

tissue

forebrain

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq: The E12.5 forebrain and newborn (P1) lenses of CD-1 mice (Charles River Laboratories) were dissected, tissue was freshly crosslinked and homogenized. Sonicated lysates were clarified and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

25989067

Reads aligned (%)

83.3

Duplicates removed (%)

12.9

Number of peaks

289 (qval < 1E-05)

mm9

Number of total reads

25989067

Reads aligned (%)

83.2

Duplicates removed (%)

13.0

Number of peaks

231 (qval < 1E-05)