Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
EED-null ES cells, input
strain/background
mixed
genotype/variation
eed fl/fl; cag-cre-ert2; tamoxifen treated
cell type
embryonic stem (ES) cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates from 2e7 cells were sonicated and clarified by centrifugation and used in IP with antibodies specified. Libraries were prepared using Kapa Hyper ChIP kit for Illumina (catalogue number KK8500).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
32637234
Reads aligned (%)
96.8
Duplicates removed (%)
25.8
Number of peaks
536 (qval < 1E-05)

mm9

Number of total reads
32637234
Reads aligned (%)
96.6
Duplicates removed (%)
25.8
Number of peaks
558 (qval < 1E-05)

Base call quality data from DBCLS SRA