Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Neutrophils

MeSH Description

Immature neutrophils.

Sample

source_name

Neutrophil, Chromatin IP against H3K27ac

cell type

Neutrophil

condition

healthy

chip antibody

Rabbit polyclonal to Histone H3 (acetyl K27) - ChIP Grade (ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was fixed in 1% formaldehyde, sheared to 100-900bp using Micrococcal nucleasean (Cell Signalling Technologies Inc.) and ultrasonic sonicator (FB-705, Fisher Scientific), immunoprecipitated using the indicated antibodies, purified and sequenced using Illumina HiSeq 2500 as recommended by the manufacturer.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

45472317

Reads aligned (%)

94.5

Duplicates removed (%)

27.4

Number of peaks

5342 (qval < 1E-05)

hg19

Number of total reads

45472317

Reads aligned (%)

93.9

Duplicates removed (%)

28.2

Number of peaks

5122 (qval < 1E-05)