Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Neutrophils
MeSH Description
Immature neutrophils.

Attributes by original data submitter

Sample

source_name
Neutrophil, Chromatin IP against H3K27ac
cell type
Neutrophil
condition
healthy
chip antibody
Rabbit polyclonal to Histone H3 (acetyl K27) - ChIP Grade (ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was fixed in 1% formaldehyde, sheared to 100-900bp using Micrococcal nucleasean (Cell Signalling Technologies Inc.) and ultrasonic sonicator (FB-705, Fisher Scientific), immunoprecipitated using the indicated antibodies, purified and sequenced using Illumina HiSeq 2500 as recommended by the manufacturer.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
41303544
Reads aligned (%)
96.2
Duplicates removed (%)
29.1
Number of peaks
8300 (qval < 1E-05)

hg19

Number of total reads
41303544
Reads aligned (%)
95.7
Duplicates removed (%)
29.9
Number of peaks
8244 (qval < 1E-05)

Base call quality data from DBCLS SRA