GSM1633748: H3K36me3 ChIP Uninvolved Kidney 3 Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Kidney
Cell type
Nephrectomy sample
NA
NA
Attributes by original data submitter
Sample
source_name
Nephrectomy sample
tissue
kidney
tissue type
uninvolved kidney
chip antibody
none (input)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to ActiveMotif instructions; ChIP and input DNAs were prepared at ActiveMotif (Carlsbad, CA) by converting overhangs into phosphorylated blunt ends, the addition of an adenine to the 3’ ends, and the addition of Illumina adaptors. The library was size-selected (175-225 base pairs [bp]) and amplified for 18 cycles.