Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9ac

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
Day 8 differentiated cells
cell type
Day 8 differentiated cells
chip antibody
H3K9Ac (Millipore)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
38640004
Reads aligned (%)
99.2
Duplicates removed (%)
6.1
Number of peaks
19904 (qval < 1E-05)

hg19

Number of total reads
38640004
Reads aligned (%)
98.5
Duplicates removed (%)
7.2
Number of peaks
19904 (qval < 1E-05)

Base call quality data from DBCLS SRA