Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Gps2

Cell type

Cell type Class
Blood
Cell type
RAW 264.7
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
RAW264.7 cell line (macrophages)
background strain
BALB/C
genotype
--
month
--
antibody
GPS2 N-terminal (home raised)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
From the cell lysates, cell nuclei were isolated, sonicated and incubated with magnetic bead-antibody complexes. The DNA samples were sent to EMBL Genomics Core Facilities (Heidelberg, Germany) for library preparation.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
28228020
Reads aligned (%)
97.8
Duplicates removed (%)
15.7
Number of peaks
2614 (qval < 1E-05)

mm9

Number of total reads
28228020
Reads aligned (%)
97.6
Duplicates removed (%)
15.8
Number of peaks
2593 (qval < 1E-05)

Base call quality data from DBCLS SRA