Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

Bone marrow derived macrophages

background strain

C57BL/6

genotype

WT

month

12-16 weeks

antibody

total input

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

From the cell lysates, cell nuclei were isolated, sonicated and incubated with magnetic bead-antibody complexes. The DNA samples were sent to EMBL Genomics Core Facilities (Heidelberg, Germany) for library preparation.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

21850401

Reads aligned (%)

96.8

Duplicates removed (%)

21.2

Number of peaks

556 (qval < 1E-05)

mm9

Number of total reads

21850401

Reads aligned (%)

96.4

Duplicates removed (%)

21.1

Number of peaks

596 (qval < 1E-05)