Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ARID3B

Cell type

Cell type Class
Others
Cell type
OECM-1
NA
NA

Attributes by original data submitter

Sample

source_name
Head and neck cancer cell line OECM1 receving Cas9-ctrl
cell line
OECM1
cell type
Head and neck cancer cell
chip antibody
ARID3B
chip antibody vendor
BETHYL

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified by the Pierce Magnetic ChIP Kit and histone-DNA complexes were isolated with antibody. 10 ng DNA was end-repaired, A-tailed and adaptor-ligated following the Illumina's TruSeq DNA preparation protocol.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
29199741
Reads aligned (%)
99.0
Duplicates removed (%)
13.1
Number of peaks
680 (qval < 1E-05)

hg19

Number of total reads
29199741
Reads aligned (%)
98.4
Duplicates removed (%)
14.2
Number of peaks
540 (qval < 1E-05)

Base call quality data from DBCLS SRA