GSM1624419: WCE in Male Setdb1+/+ X/Y MEFs replicate 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
source_name
mouse embryonic fibroblasts
strain
129/C57 dams x CAST sires
tissue
E13.5 mouse embryo derived fibroblasts
genotype
Setdb1+/+ X/Y
shRNA
Nonsilencing
sequencing protocol
single-end
chip antibody
N/A
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, nuclei were isolated from formaldehyde crosslinked NSCs and chromatin was fragmented by sonication. Chromatin immunoprecipitation was performed with corresponding antibodies. DNA was extracted from the immunoprecipitated fraction following reverse-crosslinking. Sequencing libraries was generated with Illumina's TruSeq DNA Sample Preparation Kit according to manufacturer's instruction.