Antigen

Antigen Class

TFs and others

Antigen

SUMO2

Cell type

Cell type Class

Prostate

Cell type

VCaP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

VCaP cells

cell line

VCaP

treatment1

heat shock (30min at 43 degree C)

chip antibody

anti-SUMO-2/3 (MBL, catalog# M114-3, lot# 024)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei from approximately 10 million formaldehyde crosslinked (1%; 10min at room temperature for VCaP cells, and 8min. at room temperature for K562 cells) cells were isolated and chromatin was fragmented using sonicator (bioruptor). Lysates were cleared and protein-DNA complexes were isolated using target specific antibodies and protein-A/G magnetic beads. DNA fragments were purified and libraries were prepared accorcing to standard Illumina protocol. Samples were sequenced at BGI Tech Solutions (Shenzhen, China) or at the Gene Core in EMBL (Heidelberg, Germany).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

84572477

Reads aligned (%)

52.8

Duplicates removed (%)

34.9

Number of peaks

29840 (qval < 1E-05)

hg38

Number of total reads

84572477

Reads aligned (%)

53.3

Duplicates removed (%)

32.6

Number of peaks

29845 (qval < 1E-05)