GSM1619986: input shLuc -Glucose; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Liver
Cell type
Hep 3B
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Hep3B cells_input
antibody
none (input)
cell line
Hep3B
shRNA
Luc
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-IT High Sensitivity kit (Active motif) according to the manufacturer’s manual Prepared using DNA Library preparation kit (KAPA, KK8232), as per the manufacturer’s protocol. In brief, DNA was fragmented to a median size of 150bp by sonication. Fragmented DNA ends were polished and 5′-phosphorylated. After addition of 3′-A to the ends, indexed Y-adapters were ligated and the samples were PCR amplified.