GSM1619984: shLuc +Glucose H3K9ac ChIPseq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9ac
Cell type
Cell type Class
Liver
Cell type
Hep 3B
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
Hep3B cells_ChIP
antibody
anti-H3K9ac, Active Motif, 39917
cell line
Hep3B
shRNA
Luc
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-IT High Sensitivity kit (Active motif) according to the manufacturer’s manual Prepared using DNA Library preparation kit (KAPA, KK8232), as per the manufacturer’s protocol. In brief, DNA was fragmented to a median size of 150bp by sonication. Fragmented DNA ends were polished and 5′-phosphorylated. After addition of 3′-A to the ends, indexed Y-adapters were ligated and the samples were PCR amplified.