Antigen

Antigen Class

TFs and others

Antigen

Sumo1

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

Bone marrow derived dendritic cells

genotype

UBC9WT

cell type

BMDC

chip antibody

SUMO1 (Y299 monoclonal antibody)

stimulation

LPS 10ng/ml 2 hours

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and SUMO1-, SUMO2-, or RNAPolII-DNA complexes were isolated with antibody, as described in Blecher-Gonen et al., Nature Protocols 2013. Libraries were prepared according to Blecher-Gonen et al., Nature Protocols 2013.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

5778707

Reads aligned (%)

95.9

Duplicates removed (%)

14.6

Number of peaks

250 (qval < 1E-05)

mm9

Number of total reads

5778707

Reads aligned (%)

95.6

Duplicates removed (%)

15.0

Number of peaks

324 (qval < 1E-05)