Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Trim33

Cell type

Cell type Class
Blood
Cell type
Leukemia, Lymphoid
MeSH Description
Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.

Attributes by original data submitter

Sample

source_name
TAL transgenic/HEB heterozygous with Notch truncations (provided by I.Aifantis, NYU)
strain
C57BL/6
cell type
B cell leukemia
antibody
Trim33 (A301-060A; Bethyl)
genotype
T-ALL

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with appopriate sizes were prepared for sequencing. These libraries were made following the instructions of illumina Truseq DNA sample prep kit v2.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
59372794
Reads aligned (%)
90.8
Duplicates removed (%)
23.3
Number of peaks
925 (qval < 1E-05)

mm9

Number of total reads
59372794
Reads aligned (%)
90.4
Duplicates removed (%)
23.1
Number of peaks
1031 (qval < 1E-05)

Base call quality data from DBCLS SRA