Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Pro-B cells

NA

NA

Sample

source_name

pro B-Cell

strain

C57BL/6

cell type

B cell leukemia

antibody

none

genotype

transformed pro B-Cell

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with appopriate sizes were prepared for sequencing. These libraries were made following the instructions of illumina Truseq DNA sample prep kit v2.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

32454943

Reads aligned (%)

97.4

Duplicates removed (%)

14.1

Number of peaks

357 (qval < 1E-05)

mm9

Number of total reads

32454943

Reads aligned (%)

97.2

Duplicates removed (%)

14.1

Number of peaks

421 (qval < 1E-05)