Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Trim33

Cell type

Cell type Class
Blood
Cell type
B-ALL
NA
NA

Attributes by original data submitter

Sample

source_name
Bcr-Abl transformed p19Arf null B-ALL
strain
C57BL/6
cell type
B cell leukemia
antibody
Trim33 (A301-060A; Bethyl)
genotype
Bcr-Abl overexpressed, p19Arf -/- (B-ALL)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with appopriate sizes were prepared for sequencing. These libraries were made following the instructions of illumina Truseq DNA sample prep kit v2.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
45534483
Reads aligned (%)
77.8
Duplicates removed (%)
51.7
Number of peaks
1166 (qval < 1E-05)

mm9

Number of total reads
45534483
Reads aligned (%)
77.0
Duplicates removed (%)
51.4
Number of peaks
1335 (qval < 1E-05)

Base call quality data from DBCLS SRA