Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D cells
cell line
ER+ Breast Cancer Cell Line T47D
passages
27-30
treatment
siCont_IL6
chip antibody
ERα ab3575 and Merck Millipore 06-935 antibody mix

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were prepared and antibody-bound chromatin was purified using appropriate antibodies. Antibodies used: ERα ab3575 and Merck Millipore 06-935 antibody mix. DNA was prepared for Illumina sequencing on the Novaseq 6000 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg19

Number of total reads
31637345
Reads aligned (%)
76.3
Duplicates removed (%)
22.6
Number of peaks
33145 (qval < 1E-05)

hg38

Number of total reads
31637345
Reads aligned (%)
77.5
Duplicates removed (%)
21.8
Number of peaks
33222 (qval < 1E-05)

Base call quality data from DBCLS SRA