GSM4705577: T47D ER ChIP-seq siCont Veh rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T47D cells
cell line
ER+ Breast Cancer Cell Line T47D
passages
27-30
treatment
siCont_Veh
chip antibody
ERα ab3575 and Merck Millipore 06-935 antibody mix
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were prepared and antibody-bound chromatin was purified using appropriate antibodies. Antibodies used: ERα ab3575 and Merck Millipore 06-935 antibody mix. DNA was prepared for Illumina sequencing on the Novaseq 6000 using the ThruPLEX DNA-seq kit according to the manufacturer's instructions.