Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

S2 cells

chipseq antibody

rabbit anti MYST5 (2)

technique

Shearing technique: Bioruptor

cell line

S2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP of MYST5 from Schneider cells (SL2, S2) was performed as described in Wu CH et al, Genes Dev 17: 1402 (2003). ChIP samples from two independent MYST5 antibodies and input samples were prepared using the NEXTflex ChIP-Seq Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

43848249

Reads aligned (%)

70.2

Duplicates removed (%)

78.8

Number of peaks

8930 (qval < 1E-05)

dm3

Number of total reads

43848249

Reads aligned (%)

70.5

Duplicates removed (%)

76.9

Number of peaks

10130 (qval < 1E-05)