Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Digestive tract

Cell type

Small intestine

NA

NA

Sample

source_name

Prdm1/Blimp-1 mutant anti-Irf-1 input

strain background

C57Bl6/J;129/SvEv

developmental stage

E18.5

genotype/variation

Prdm1/Blimp-1 mutant

tissue

small intestine

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were precleared with Protein G Dynabeads. Complexes were then isolated with 10µg anti-GFP monoclonal antibody (Invitrogen A11120, clone 3E6, IgG2a), polyclonal rabbit anti-Irf1 antibody (M-20, Santa Cruz, SC-640) or control rabbit IgG (Santa Cruz sc-2027, Mouse IgG) linked to Protein G Dynabeads. Sequencing libraries were then prepared using the Illumina ChIP-seq DNA Sample Prep Kit. Libraries were size selected at ~200bp and four libraries were multiplexed per lane on a HiSeq 2000.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

26093745

Reads aligned (%)

99.0

Duplicates removed (%)

11.3

Number of peaks

274 (qval < 1E-05)

mm9

Number of total reads

26093745

Reads aligned (%)

98.8

Duplicates removed (%)

11.9

Number of peaks

247 (qval < 1E-05)